Purpose:
The purpose of this SOP is to provide written guidelines
for the use of Colony Counter.
Definition
CFU: The unit is used to specify the number of
colonies obtained in the test sample.
Isolates: Is used to identify the culture
type, character of colonies obtained in environmental condition.
Procedure:
Implementation:
- Colony counter is used to count the colony forming unit. (CFU).
- Switch ON the mains.
- Clean the lens & specimen stage.
- Place the plate on specimen stage & confirm that the display shows ‘ZERO’ count.
- Take the SENSOR in hand & start marking the colonies of plates.
- On every marking SENSOR will beep once & finally total colonies marked by SENSOR on plate will be displayed on screen.
- To check another sample RESET the machine & repeat the procedure as above.
- If SENSOR is not working you can count the colonies manually.
- Before counting manually check that the count is ‘ZERO’.
- While counting the colonies by manual method instead of marking with SENSOR we have to count the colony & press MANUAL for one colony.
- There will be one beep for one colony.
- After completion of counting the total no. of colonies will be displayed on screen.
- Clean the lens of specimen stage.
Precautions
Ensure that the equipment is cleaned properly before
use.
